Genetic diversity and evolvement of natural populations in Aquilaria sinensis (Lour.) Gilg
Meiling Zou1, Zhiqiang Xia1, Cheng Lu1, Haiyan Wang1, Jiaming Ji1 and Wenquan Wang1* 1Institute of Tropical Bioscience and Biotechnology/ State Key laboratory of Tropical Crop Biotechnology, CATAS, Haikou 571101, China
Aquilaria sinensis (Lour.) Gilg, one of the valuable medicinal plants uniquely native in Southern China, has been over destroyed, and to be endangered species listed in China Plants Red Book. Levels of genetic diversity and evolvement in Aquilaria sinensis (Lour.) Gilg were investigated using 20 ISSR and 20 SRAP markers. Forty primers produced a total of 567 polymorphic alleles used to evaluate genetic variation and evolvement. I (0.3582) and h (0.2397) values indicate a narrow variation among the 7 populations examined, indicated that it was reaching or endangered state theoretically, and need pay attention to conserve. The genetic diversity index in Jianfengling, Bawang and Guangdong populations are higher than others (0.2954, 0.2859,0.2938), showing that they could be the hot spot to take the in situ conservation. The distance UPGMA tree grouped together mainly according to their geography origin, with some of the crosses. PCA analysis further shown that there are three large scale level geographic distribution zones of Aquilaria sinensis (Lour.) Gilg: Hainan group, Guangdong group and among them of Diaoloushan group. 21.47% of total genetic variation existed among 7 populations and gene flow among populations was relatively restricted (Nm=1.8292). According to evolutionary analysis, it is indicated that, some of the Diaolushan, Wuzhishan and Limushan accessions were first branched among the 11 natural populations. The latest branched Jianfengling and Maogan accessions are the younger developed populations with higher diversity.
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