Genetic analysis of QTLs associated with cold tolerance during seedling stage in rice
Guo Zong1, Ahong Wang1, Lu Wang1, Tao Sang1,2, and Bin Han1,*
1
National Center for Gene Research and Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China
2
Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824, USA
Rice (Oryza sativa L.) is one of the most important crops in the world. Low temperature stress is a common problem of rice cultivation, and is a significant factor affectting global food production. Previous investigations based on the>[5] C.C. Li and J.N. Rutger, Inheritance of cool-temperature seedling vigor in rice and its relationship with other agronomic characters, Crop. Sci. 20 (1980), pp. 295–298.. With the successful application of QTL mapping technology in many plant species over the past decade, more and more genes (or QTLs) affecting a wide range of agriculturally and biologically important complex traits have been identified. As cold tolerance is so important for rice, we suppose that more than one major QTLs are responsible for it. So we constructed a skeleton bin map with a total of 2334 recombination bins using 150 RILs derived from crosses between Nipponbare (japonica, cold tolerant) and 93-11 (indica, cold sensitive) to investigate rice cold tolerance at seedling stage. Then three QTLs were detected on chromosome 2, 4 and 12. And these QTLs may be useful as targets for varietal improvement and positional cloning.
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